Fundamentals and applications of single-molecule FRET in biophysical research
Hörsaalgebäude II, Hörsaal 2 Single-molecule FRET (smFRET), a molecular ruler based on dipole-dipole coupling of two fluorescent dyes in proximity of 2-10 nm, has become an established tool to study b [...] here introduce the principles of the technique with an overview of state-of-the-art applications[1,2], highlighting its role in assessing fundamental biophysical processes such as the temporal coupling [...] confocal-, video- and super-resolution microscopy[4]. [1] Lerner et al., Science 359 (2018) eaan1133 [2] Agam et al., Nature Methods 20 (2023) 523-535 [3] Han et al., eLife (2024) in press [4] Moya et al …